|
|
| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
21/06/2023 |
Actualizado : |
21/06/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
RODRIGUEZ, N.E.; BEYHAUT, E.; SICARDI, M.; RODRÍGUEZ-BLANCO, A. |
Afiliación : |
N. E. RODRIGUEZ RODRIGUEZ, Depto. de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; ELENA BEYHAUT GUTIERREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; M. SICARDI, Instituto de Ecología y Ciencias Ambientales, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; A. RODRÍGUEZ-BLANCO, Depto. de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay. |
Título : |
Bioprospection of naturalized soybean-nodulating Bradyrhizobium strains in Uruguayan soils: a genetic and symbiotic approach. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Environmental Sustainability. 2023, volume 6, pages 161-171. https://doi.org/10.1007/s42398-022-00258-1 |
ISSN : |
2523-8922 (electronic). |
DOI : |
10.1007/s42398-022-00258-1 |
Idioma : |
Inglés |
Notas : |
Article history: Received 31 May 2022; Revised 29 September 2022; Accepted 6 December 2022; Published online 5 January 2023. -- Correspondence: A. Rodríguez-Blanco. Depto. de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay, email: andrearb@fagro.edu.uy -- |
Contenido : |
ABSTRACT.- Soybean (Glycine max (L.) Merr.), an economically relevant crop, establishes a symbiotic association with rhizobia to obtain nitrogen (N2) from the air by biological nitrogen fixation with important benefits. In Uruguay inoculants formulated with two strains, Bradyrhizobium elkanii U1301 and U1302, are recommended since 1984. Besides that, the study of native-naturalized rhizobia populations is relevant because these strains could compete with applied inoculants and may present a better symbiotic efficiency. The aim of this work was to study, genetically and symbiotically, naturalized soybean nodulating rhizobia isolated from Uruguayan soils. A collection of ten naturalized rhizobia was studied and compared with Uruguay's commercial strains and neighbouring countries (B. elkanii U1301 and U1302, Bradyrhizobium japonicum E109 and Bradyrhizobium diazoefficiens SEMIA5080). Using a multilocus sequence analysis (MLSA) (16S rRNA, atpD, gyrB and rpoB genes), five naturalized strains were identified as B. elkanii and four as B. japonicum. The other naturalized strain UYS-CA02 is suggested to belong to Bradyrhizobium ferriligni, considering a second MLSA with 16S rRNA, gyrB, rpoB, dnaK and recA genes. Analysis of symbiotic genes (nodY/K and nifH) indicates that strains U1301 and U1302 may have transferred these genes horizontally to strain UYS-CA02 or its ancestor. Symbiotic efficiency was evaluated in axenic conditions, in which shoot dry weight, total nitrogen in shoots, number of nodules and nodules dry weight, were determined. In that assay, the U1301:U1302 blend outstood in front of other commercial strains. Multivariate analysis of symbiotic efficiency data shows a better performance of B. elkanii-like strains than B. japonicum-like ones. The Author(s) under exclusive licence to Society for Environmental Sustainability 2023. MenosABSTRACT.- Soybean (Glycine max (L.) Merr.), an economically relevant crop, establishes a symbiotic association with rhizobia to obtain nitrogen (N2) from the air by biological nitrogen fixation with important benefits. In Uruguay inoculants formulated with two strains, Bradyrhizobium elkanii U1301 and U1302, are recommended since 1984. Besides that, the study of native-naturalized rhizobia populations is relevant because these strains could compete with applied inoculants and may present a better symbiotic efficiency. The aim of this work was to study, genetically and symbiotically, naturalized soybean nodulating rhizobia isolated from Uruguayan soils. A collection of ten naturalized rhizobia was studied and compared with Uruguay's commercial strains and neighbouring countries (B. elkanii U1301 and U1302, Bradyrhizobium japonicum E109 and Bradyrhizobium diazoefficiens SEMIA5080). Using a multilocus sequence analysis (MLSA) (16S rRNA, atpD, gyrB and rpoB genes), five naturalized strains were identified as B. elkanii and four as B. japonicum. The other naturalized strain UYS-CA02 is suggested to belong to Bradyrhizobium ferriligni, considering a second MLSA with 16S rRNA, gyrB, rpoB, dnaK and recA genes. Analysis of symbiotic genes (nodY/K and nifH) indicates that strains U1301 and U1302 may have transferred these genes horizontally to strain UYS-CA02 or its ancestor. Symbiotic efficiency was evaluated in axenic conditions, in which shoot dry weight, total nitrogen in shoots,... Presentar Todo |
Palabras claves : |
Biological nitrogen fixation; Bradyrhizobium; Glycine max; MLSA; Phylogeny; PLATAFORMA DE BIOINSUMOS - INIA; Rhizobia. |
Asunto categoría : |
P01 Conservación de la naturaleza y recursos de La tierra |
Marc : |
LEADER 03065naa a2200277 a 4500 001 1064205 005 2023-06-21 008 2023 bl uuuu u00u1 u #d 022 $a2523-8922 (electronic). 024 7 $a10.1007/s42398-022-00258-1$2DOI 100 1 $aRODRIGUEZ, N.E. 245 $aBioprospection of naturalized soybean-nodulating Bradyrhizobium strains in Uruguayan soils$ba genetic and symbiotic approach.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 31 May 2022; Revised 29 September 2022; Accepted 6 December 2022; Published online 5 January 2023. -- Correspondence: A. Rodríguez-Blanco. Depto. de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay, email: andrearb@fagro.edu.uy -- 520 $aABSTRACT.- Soybean (Glycine max (L.) Merr.), an economically relevant crop, establishes a symbiotic association with rhizobia to obtain nitrogen (N2) from the air by biological nitrogen fixation with important benefits. In Uruguay inoculants formulated with two strains, Bradyrhizobium elkanii U1301 and U1302, are recommended since 1984. Besides that, the study of native-naturalized rhizobia populations is relevant because these strains could compete with applied inoculants and may present a better symbiotic efficiency. The aim of this work was to study, genetically and symbiotically, naturalized soybean nodulating rhizobia isolated from Uruguayan soils. A collection of ten naturalized rhizobia was studied and compared with Uruguay's commercial strains and neighbouring countries (B. elkanii U1301 and U1302, Bradyrhizobium japonicum E109 and Bradyrhizobium diazoefficiens SEMIA5080). Using a multilocus sequence analysis (MLSA) (16S rRNA, atpD, gyrB and rpoB genes), five naturalized strains were identified as B. elkanii and four as B. japonicum. The other naturalized strain UYS-CA02 is suggested to belong to Bradyrhizobium ferriligni, considering a second MLSA with 16S rRNA, gyrB, rpoB, dnaK and recA genes. Analysis of symbiotic genes (nodY/K and nifH) indicates that strains U1301 and U1302 may have transferred these genes horizontally to strain UYS-CA02 or its ancestor. Symbiotic efficiency was evaluated in axenic conditions, in which shoot dry weight, total nitrogen in shoots, number of nodules and nodules dry weight, were determined. In that assay, the U1301:U1302 blend outstood in front of other commercial strains. Multivariate analysis of symbiotic efficiency data shows a better performance of B. elkanii-like strains than B. japonicum-like ones. The Author(s) under exclusive licence to Society for Environmental Sustainability 2023. 653 $aBiological nitrogen fixation 653 $aBradyrhizobium 653 $aGlycine max 653 $aMLSA 653 $aPhylogeny 653 $aPLATAFORMA DE BIOINSUMOS - INIA 653 $aRhizobia 700 1 $aBEYHAUT, E. 700 1 $aSICARDI, M. 700 1 $aRODRÍGUEZ-BLANCO, A. 773 $tEnvironmental Sustainability. 2023, volume 6, pages 161-171. https://doi.org/10.1007/s42398-022-00258-1
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas. |
Fecha actual : |
21/02/2014 |
Actualizado : |
06/11/2018 |
Tipo de producción científica : |
Boletín de Divulgación |
Autor : |
ARBOLEYA, J.; CAPRA, G.; ALBIN, A. (Ed.). |
Afiliación : |
JORGE EDUARDO ARBOLEYA DUFOUR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUSTAVO ENRIQUE CAPRA CASSINONI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALFREDO SANTIAGO ALBIN FERREIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Producción de cebolla en la zona sur. |
Fecha de publicación : |
1993 |
Fuente / Imprenta : |
Montevideo (Uruguay): INIA, 1993. |
Páginas : |
91 p. |
Serie : |
(INIA Boletin de Divulgación; 29) |
ISBN : |
9974-556-54-6 |
ISSN : |
1510-7396 |
Idioma : |
Español |
Thesagro : |
CEBOLLA; COMERCIALIZACIÓN; CONTROL DE MALEZAS; COSTOS DE PRODUCCION; CULTIVO; ENFERMEDADES DE LAS PLANTAS; ESTUDIO DE MERCADOS; FERTILIZACION; MANEJO DEL CULTIVO; PLAGAS DE LAS PLANTAS; PRODUCCION; SISTEMAS DE PRODUCCIÓN. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/2726/1/111219240807155055.pdf
|
Marc : |
LEADER 00840nam a2200313 a 4500 001 1012036 005 2018-11-06 008 1993 bl uuuu u00u1 u #d 020 $a9974-556-54-6 022 $a1510-7396 100 1 $aARBOLEYA, J. 245 $aProducción de cebolla en la zona sur. 260 $aMontevideo (Uruguay): INIA$c1993 300 $a91 p. 490 $a(INIA Boletin de Divulgación; 29) 650 $aCEBOLLA 650 $aCOMERCIALIZACIÓN 650 $aCONTROL DE MALEZAS 650 $aCOSTOS DE PRODUCCION 650 $aCULTIVO 650 $aENFERMEDADES DE LAS PLANTAS 650 $aESTUDIO DE MERCADOS 650 $aFERTILIZACION 650 $aMANEJO DEL CULTIVO 650 $aPLAGAS DE LAS PLANTAS 650 $aPRODUCCION 650 $aSISTEMAS DE PRODUCCIÓN 700 1 $aCAPRA, G. 700 1 $aALBIN, A.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|